While majority of subpatent infections are due to low density parasitaemia; in situations where HRP2-based RDTs are used, deletion of the histidine-rich protein 2 and histidine-rich protein 3 genes (pfhrp2 and pfhrp3) has also been implicated as a reason for the failure of RDTs to detect P. falciparum even with higher density infections [57–59]. Here, HDGFL2 is linked to infection.