WT1-CTLs from all 3 donors tested displayed significant cytotoxicity against the 3 AML cell lines at both low and high effector:target (E:T) ratios, with <50% of AML cells remaining viable at an E:T of 0.5 and <10% alive at an E:T of 5 (Fig. 1D, Supplementary Fig. 1E, F). Here, WT1 is linked to acute myeloid leukemia.