In addition, the expression of DNMTs (DNMT1, DNMT3a and DNMT3b) was quantified because they encoded the main enzymes involved in the methylation of DNA and previously we have detected a regulation of these genes after following a VLCKD in blood leukocytes of patients with obesity [11]. This evidence concerns the gene DNMT3B and obesity due to melanocortin 4 receptor deficiency.