The TWAS analysis showed that overexpression of SUN2, JOSD1, and CBY1 was negatively associated with the risk of cholelithiasis and GERD in the blood and esophagus-related tissues, while overexpression of JOSD1 and CBY1 was positively associated with these 2 diseases in the liver tissue. The gene discussed is JOSD1; the disease is cholelithiasis.