We sorted engineered CD90.1+CTLs (Akt1-OE or Akt2-OE CTLs) or CD90.1+GFP+ CTLs (NFAT1OEAkt2-OE CTLs) from the in vitro CTL culture and adoptively transferred them into HCC-bearing mice. The gene discussed is AKT1; the disease is hepatocellular carcinoma.