To further demonstrate the utility of the ASFV synthetic genomics reverse genetics system, recombinant viruses containing C-terminal fusions of mCherry with CD2v (CD2v-mCh/p12M) or Cerulean with K145R (K145R-Cer/p12M) were generated since they can be used for live-cell imaging to facilitate functional characterization during infection. Here, PMCH is linked to infection.