Relevant studies have shown that neferine can activate the iron death-related signaling pathway Nrf2/HO-1/NQO1 to induce iron death in thyroid cells and inhibit their proliferation [29]; inhibit colon cancer cells by activating p65 and STAT3 [30] and they can activate the p38 and MAPK/JUK signaling pathways to induce the proliferation and migration of prostate cancer cells [30].Meanwhile, related studies have shown that neferine can also induce apoptosis in prostate cancer cells through autophagic flux and the JUK pathway [32]. This evidence concerns the gene HMOX1 and malignant colon neoplasm.