HOXB13 and diffuse midline glioma: On the other hand, no nuclear HOXB13 expression was detected in 15 SP-EP, four spinal subependymomas (SP-SE), five spinal ependymomas with MYCN amplification (SP-EP-MYCN), four spinal diffuse midline glioma with H3 K27 alteration, four spinal pilocytic astrocytomas, and one rosette-forming glioneuronal tumor.