On the other hand, no nuclear HOXB13 expression was detected in 15 SP-EP, four spinal subependymomas (SP-SE), five spinal ependymomas with MYCN amplification (SP-EP-MYCN), four spinal diffuse midline glioma with H3 K27 alteration, four spinal pilocytic astrocytomas, and one rosette-forming glioneuronal tumor. This evidence concerns the gene HOXB13 and mixed neuronal-glial tumor.