Through the application of tomato hairy root and virus-induced gene silencing (VIGS) technologies for the investigation of lncRNA47258, it was determined that lncRNA47258 could target the TCP (Solyc07g062681.1) gene and modulate the metabolic pathway of tomato jasmonic acid-related indices, thereby impeding the infection of M. incognita. This evidence concerns the gene SPINK1 and infection.