Owing to the moderate affinity of NOTA-GC-PSMA, the tumor site of LNCaP tumor-bearing mice showed specific uptake of 68Ga-NOTA-GC-PSMA (3.10 ± 0.20 %ID/g at 30 min and 2.28 ± 0.27 %ID/g at 60 min). This evidence concerns the gene FOLH1 and neoplasm.