INS and Hyperinsulinemia: Figure 1A–D shows typical experiments, and Figure 1E,F summarizes the results. Figure 1 shows treatment with a high concentration of insulin-induced increase in cells (Figure 1A–E) and nuclear (Figure 1A–C,F). This effect was highly significant (p < 0.0001) (Figure 1E,F). In addition, as shown in Figure 1A–F, hyperinsulinemia did not change the difference in volume between VSMCs from men and women.