Experimental results demonstrate that the biosensor enables sensitive detection of cytokines (e.g., TNF-α and IL-6), representative cervical carcinoma severity biomarkers, in undiluted physiological media (e.g., artificial sweat and lavage fluid) with limits of detection (LOD) as low as 0.13 pM for TNF-a and 0.20 pM for IL-6. The gene discussed is TNF; the disease is cervical carcinoma.