By using multiplex immunofluorescence (mIF), a technique which allows the detection and quantification of both cellular expression and co-localization of proteins in a tissue specimen [50], Wang et al. have shown that the expression of CD276 and Iba1 are correlated in glioma (p < 0.001, R2 = 0.13) [51]. Here, AIF1 is linked to glioma.