LRS-based CAH genotyping assay has been established and consists of 6 locus-specific long-range PCR (LR-PCR) fragments to cover the entire gene regions and exon–intron boundary regions for CYP21A2, CYP11B1, CYP17A1, HSD3B2, and StAR genes [17]. Here, CYP21A2 is linked to congenital adrenal hyperplasia.