Further analysis showed overexpression of LINC00261, using a lentiviral vector (LV-LINC00261) in colon cancer cell lines HCT-116 and DLD-1, inhibits tumor progression by sponging the oncogenic miR-324-3p to activate GSK-3β (glycogen synthase kinase 3β), thus degrading β-catenin and inhibiting the Wnt/β-catenin pathway and resulting in decreased proliferation, migration, invasion, apoptosis enhancement, and decreased tumor growth both in vitro and in vivo. This evidence concerns the gene GSK3B and neoplasm.