In this study, the effects of the DOT1L inhibitor EPZ004777 on the NPM1-mutant AML cell lines AML2 and AML3 were evaluated by analyzing RNA sequencing data obtained from the NCBI-Gene Expression Omnibus (GEO) database titled ‘Targeting Chromatin Regulators: Inhibition of Leukemogenic Gene Expression in NPM1 Mutant Leukemia’ (accession code: GSE85107). This evidence concerns the gene DOT1L and acute myeloid leukemia.