The screening assay results unveiled that the expression of IL-1β and TNF-α mRNA (Fig. 1F and G), the maturation of IL-1β (p17), and the cleavage of Caspase-1 (p20) (Fig. 1H) in ASFV-ΔI177L-infected cells were lowest compared with those in the parental ASFV-infection group, demonstrating the impact of ASFV-ΔI177L on modulating inflammatory responses and the crucial role of ASFV I177L in ASFV-mediated inflammation. This evidence concerns the gene TNF and infection.