To assess the impact of HSV-1 infection on MAIT cell viability, expression of the intracellular apoptotic effector protein, cleaved caspase-3 (CC3), together with amine-reactive LIVE/DEAD viability dye (LD) staining, was measured in MAIT cells using flow cytometry after 16 hours of infection. The gene discussed is CASP3; the disease is infection.