To investigate the role of Fis1 in hyperglycemia‐induced vascular endothelial injury, ApoE−/− mice were injected via the tail vein with AAV9 (recombinant adeno‐associated virus 9) containing Fis1 shRNA, driven by the endothelial cell‐specific Tie promoter. Here, TIE1 is linked to Hyperglycemia.