Nowadays, most sequencing studies of chordoma, including our previous one, mainly focus on the genome.[7, 11] Nevertheless, in addition to gene mutations, alterations in mRNA and protein also contribute to oncogenesis and cancer progression, which remain elusive in chordoma.[12] In this study, via transcriptome and proteome analyses and in‐depth functional studies, we identify RAB3B as a prominent tumorigenic regulator in chordoma with high transcriptional activity and prognostic value. Here, RAB3B is linked to cancer.