KCNJ11 and pancreatic insulinoma: To minimize protein expression variations and increase screening efficiency, we transduced rat insulinoma cells INS-1 in a large culture dish with recombinant adenoviruses encoding SUR1F27S (from golden hamster Mesocricetus auratus) and wild-type (WT) Kir6.2 (from rat Rattus rattus), and then divided the transduced cells into multi-well tissue culture plates for compound testing (see Methods and Figure 1—figure supplement 1A).