RT‐PCR analysis (Figure 4B) of melanoma cell lines A375 and A375 MEK after treatment with IC50 concentrations of KU757 confirmed the downregulation of CD20, CD25 and NDUFA7, as well as the upregulation of PRKCH, BMP2 and ADAMTS9 hub genes (p < 0.05). The gene discussed is NDUFA7; the disease is melanoma.