Especially seven significantly enriched metabolic pathways including PI3K-Akt signaling pathway, MAPK signaling pathway, shigellosis, salmonella infection, Escherichia coli infection, legionellosis and endocytosis overrepresented the metabolic direction of most proteins (p < 0.05) (Figure 2C; Supplementary Table S2). The gene discussed is AKT1; the disease is escherichia coli infection.