ACSS2 and cancer: Oncogenic and growth factor signaling in cancer cells increases mTORC1 signaling, resulting in the cleavage and translocation of SREBPs from the endoplasmic reticulum to the nucleus, where they upregulate genes for de novo lipid and cholesterol synthesis.30 SREBPs play a crucial role in regulating genes encoding ATP-citrate lyase (ACLY), ACSS2, and acetyl-CoA carboxylase (ACC), among others that drive lipogenesis.27,28,31 Western blot analysis was performed to evaluate the influence of KRAS mutations on the expression of key proteins driving lipogenesis.