KRAS and neoplasm: When acetyl-CoA is needed, citrate is cleaved by the ACLY to generate oxaloacetate and acetyl-CoA.32 In tumor cells, an alternative route converts cytoplasmic acetate to acetyl-CoA using ACSS2.33,34 Western blot analysis revealed no significant differences in the phosphorylation of ACLY; however, the expression of ACSS2 was significantly elevated in KRAS G12V cells under low-serum conditions and remained elevated after growth factor stimulation (Figures 4A, 4B, and S4A).