Since both kRasG12V and Ventx have been reported to play a role in brain cancer (Lam et al., 2022), we decided to activate the oncogene kRasG12V at 1 dpf in a single normal cell of the brain of a transgenic zebrafish (injected with the mRNA of Ventx-GR at one-cell stage). Here, VENTX is linked to brain cancer.