The functional assay revealed that HMGB1 overexpression markedly increased the proliferative abilities of DR882MUT AML and circKCNQ5 silencing, whereas HMGB1 downregulation remarkably inhibited the proliferative abilities of DNMT3A-WT AML and circKCNQ5 overexpression. Here, DNMT3A is linked to acute myeloid leukemia.