In addition, HMGB1 protein levels were significantly enhanced in DNMT3A-WT AML cells after circKCNQ5 overexpression and significantly reduced in DNMT3A-MUT AML cells after circKCNQ5 downexpression, whereas HMGB1 mRNA had no significant change (Figure 4C and D). Here, DNMT3A is linked to acute myeloid leukemia.