ACTR6 and hepatocellular carcinoma: Through RT-qPCR we found that the expression level of ACTR6 in the three HCC cell lines was higher than that in normal liver cells (Figure 5A) Furthermore, to confirm the expression of ACTR6 in hepatocellular carcinoma and its prognostic effect, we used immunohistochemical methods to detect the protein expression of ACTR6 in 90 pairs (six pairs of which were lost) of formalin-fixed paraffin-embedded (FFPE) liver tissue samples (Figure 5B).