Target: GPC2Expression: NB PDXs and cell line xenografts with a range of GPC2 cell-surface expression:(GPC2Hi and GPC2UltraHi cell populations)In vitro: SK-N-SH/NBL-SNB cells showed higher levels of GPC2 expression which correlated with an acquired susceptibility to the D3-GPC2-PBD ADC (IC50 = 1.81 ± 1.33 pM)In vivo: evaluation of the efficacy of the ADC in NB PDXs and cell line xenografts with a range of GPC2 cell-surface expression and genomic aberrations. The gene discussed is GPC2; the disease is neuroblastoma.