Target: GPC2Expression: GPC2 stably overexpressed in NXS2-GPC2 and 9464D-GPC2 by flow cytometry. Overexpression was maintained after NXS2-GPC2 and 9464D-GPC2 in vivo tumor engraftment in immunocompetent syngeneic A/J and C57BL/6 miceIn vitro: cell viability assessment in GPC2-transduced murine NB cellsIn vivo: vaccinated subcutaneously with 2×106 9464D-GPC2 cells treated with ADC (30 ng/mL for 96 hours), rechallenged subcutaneously in the contralateral flank 7 days layer with 2×106 naïve 9464D-GPC2 cells. This evidence concerns the gene GPC2 and neoplasm.