Tnfr1 deficiency and TrifLps2 mutation protected macrophages from cell lysis at 3 h (Fig. EV1C) and 5 h (Fig. 1E) post-infection and these cells displayed reduced cleavage of caspase-8, caspase-3, GSDMD (Fig. 1F) as well as the fluorogenic caspase-3/7 substrate (Fig. EV1D) compared to WT macrophages. Here, GSDMD is linked to infection.