Consistent with our earlier observation (Fig. 5A,B), loss of NleF alone did not suppress ΔespL-induced necroptosis, since macrophage lysis following ΔnleF/espL infection remains sensitive to GSK’872 inhibition (Fig. EV5C), and MLKL phosphorylation upon ΔespL or ΔnleF/espL infection was identical (Fig. EV5D). Here, MLKL is linked to infection.