While ΔespL infection triggered comparable MLKL phosphorylation between WT, Tnfr1−/− and TrifLps2 macrophages (Fig. 4A), caspase-1 processing, GSDMD cleavage (Fig. 4A) and macrophage lysis (Fig. 4B) were reduced in TrifLps2 macrophages compared to WT and Tnfr1−/− macrophages. The gene discussed is GSDMD; the disease is infection.