In our integrated analysis, we discovered a unique subpopulation of tumor cells (MP3 cluster) in D‐TGCT that could regulate differentiation of CD34+ Fbs into MMP3+ Fbs and APOE+ Fbs through the COL6A3 − (ITGAV + ITGB8) ligand–receptor pair. Here, COL6A3 is linked to neoplasm.