In a study conducted on an animal model of CKD (induced by nephrectomy), 21 days after surgery, animals were exposed to a single dose of DOTA, showing, 2 days later, a significant decrease in total iron binding capacity and a significant increase in transferrin saturation, as well as a trend towards increased iron levels, which indicates higher iron availability; an increase in ferritin values was also described [18]. Here, TF is linked to chronic kidney disease.