We hypothesized whether TZP modulated glycolysis by targeting HIF‐1α, as it is considered a key regulator in cancer cell glucose metabolism[18] and controls the transcription of glycolysis‐related genes.[19] Immunohistochemistry results indicated that TZP reduced the expression of HIF‐1α, PFKFB3, and PFK‐1 in CRC tissues in vivo (Figure 6A,B). This evidence concerns the gene PFKM and cancer.