Since NCBP2 was correlated with several immune-related pathways, such as IFN-alpha response and TNF alpha signaling-via NFKB (Fig. 4), we further explored the correlations between NCBP2 and tumor microenvironment through Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) algorithm, which was performed to calculate the level of tumor stromal cells, and the infiltration immune cells in tumor tissues based on RNA sequencing data. The gene discussed is TNF; the disease is neoplasm.