To evaluate the effects of type I IFNs during B. pertussis infection on adaptive immune responses during B. pertussis infection, we examined pertussis toxoid (PT), filamentous hemagglutinin (FHA), and pertactin (PRN)-specific antibodies in the serum and cytokines in the supernatant of pulmonary and/or splenic lymphocytes after 3 days of incubation with PT, FHA, and PRN. Here, F2 is linked to pertussis.