To ensure that the lack of peripheral neuropathy phenotypes following hGlyRS-K456Q expression is not attributable to reduced hGlyRS-K456Q protein levels, we performed western blotting using heads of flies expressing hGlyRS transgenes ubiquitously from the adult stage onwards, to circumvent developmental lethality (tubulin-GAL80ts; tubulin-GAL4, see Materials and Methods for details). The gene discussed is LGALS4; the disease is peripheral neuropathy.