KL and cardiac hypertrophy: Furthermore, In the rat model, exogenous Klotho restored sirtuin1 (SIRTI) reduction and minimized the level of cardiac hypertrophy indicators, whereas, in H9c2 cells, it reduced ROS generation, prevented apoptosis, and improved mitochondrial dysfunction [214]. It has been demonstrated that exogenous injection of recombinant Klotho reduces lung fibrosis ex vivo by regulating the expression of known fibrotic genes, extracellular matrix formation, and bronchial fibroblast stimulation [215].