Figure 7D and E show significantly increased Her2-specific dextramer-positive CD8+ T cells and IFN-γ-producing cells in the spleen in the treated mice (tumor-free mice after triple combination treatment). These results indicate that combining TLIF-PTT+FlaB Vax with anti-PD-1 leads to very long-lasting antigen-specific CD8+ memory T cells maintaining IFN-γ-producing ability. Here, CD8A is linked to neoplasm.