,116 Additionally, among OVAT-enriched motifs, we observed upregulated expression of TFs such as GATA5, GATA6, KLF5, and WT1, consistent with their known roles in visceral fat development and adipocyte differentiation.117, 118, 119, 120 Importantly, effect directions of upregulated TFs such as EMX2 (in SAT) and GATA5, GATA6, KLF5, and WT1 (in OVAT) were validated in three validation cohorts confirming and underlining their role in obesity and metabolic dysfunction. This evidence concerns the gene KLF5 and obesity due to melanocortin 4 receptor deficiency.