Similar results in luciferase activity were observed upon transfection of pmirRB-SIK1-WT or pmirRB-SIK1-MUT into HUVECs treated with Huh7-miR-183-5p inhibitor/EVs or Hep3B-miR-183-5p mimics/EVs (Figure 5F), thereby confirming that SIK1 in HUVECs is a direct target of miR-183-5p of HCC cell-derived EVs. Here, SIK1 is linked to hepatocellular carcinoma.