In the myocardial hypoxia/reoxygenation model, this acute injury process triggers a series of cellular responses and can effectively simulate the physiological changes observed in clinical patients with acute myocardial infarction who undergo revascularization (such as thrombolysis or coronary intervention).In this model, we reduced the expression of RPS11 through gene knockdown and observed a subsequent decrease in the LC3II/I (Microtubule-associated protein 1 light chain 3 II/I)ratio and BNIP3 (BCL2 protein-interacting protein 3) expression. Here, BNIP3 is linked to acute myocardial infarction.