Breast tumor organoids derived from three independent triple negative breast cancer patients (T3N0 grade 3, T2N3 grade 3, T4N2a grade 3) were assessed for endogenous expression of circPVT1, miR-33a-5p, MYC and GLS which resulted comparable to that of the originating breast cancer tissues (Fig. 6D). The gene discussed is GLS; the disease is triple-negative breast carcinoma.