FOXP3 and obesity due to melanocortin 4 receptor deficiency: C57Bl/6J Foxp3GFP reporter mice, which are more prone to diet-induced obesity than Balb/c mice (Supplementary Fig. 1e, f), likewise had significantly reduced frequencies of hypothalamus-residing Foxp3+GFP+Tregs when maintained on the HFHS diet (Fig. 2p).