The myeloid transcriptional factors RUNX1, SPI1, GFI1, PPARG,LBR, and CITED2 were upregulated in expression in Vav-cre Tet2fl/flTp53fl/fl LSK cells compared with other groups of cells (Figure 5A, Supplemental Figure 2A, and Supplemental Table 1), and gene set enrichment analysis (GSEA) revealed an enrichment in gene signatures in LSK cells related to myeloid differentiation and AML transformation (such as immortalized HOXA9, MEIS1_up, GATA2 targets-up, and myeloid cell development up) (Supplemental Figure 2B). Here, SPI1 is linked to acute myeloid leukemia.