CD63 and type 2 diabetes mellitus: Representative TEM images of negative staining or CD63 gold-labeled EVs derived from H-RBCs (H RBC-EVs) and T2D-RBCs (T2D RBC-EVs) are shown in Figure 1A. Most of the EVs appeared intact, and the TEM pictures show the presence of spherical, membrane-capsuled structures ranging between 50 and 300 nm in diameter (Figure 1A).