To further elucidate the role of arginase-1 carried by the EVs in the development of endothelial dysfunction, we coincubated mouse aortic rings with T2D RBC-EVs in the presence or absence of the arginase inhibitor 2(S)-amino-6-boronohexanoic acid (ABH). The gene discussed is ARG1; the disease is type 2 diabetes mellitus.