Multivariate Cox analysis revealed that differentiation, tumor size, gallstones, TNM stage, invasion, lymph node metastasis, surgical procedure, and positive ASPH expression were correlated with overall survival in SC/ASC (Table 3); differentiation, tumor size, TNM stage, lymph node metastasis, invasion, surgical procedure, and positive ASPH expression were correlated with overall survival in AC patients. The gene discussed is ASPH; the disease is gallstones.