Downregulation of lncRNA-NEAT1 expression can prevent glioma cells from proliferating, and the mechanism is that after down-regulating the level of lncRNA-NEAT1 expression, the G0/G1 phase of the cell cycle of glioma U251 and LN229 cells is significantly accumulated, on the contrary, the proportion of cells in S phase is reduced. The gene discussed is NEAT1; the disease is glioma.