In addition to the identified negative correlation between the expression of CD276 and TIL scores, the remaining 8 ICGs (PDCD1, CD274, CTLA4, LAG3, TIGIT, HAVCR2, BTLA, C10orf54) displayed significant positive correlations with TIL scores across most cancer types (Pearson P < 0.05), with the highest correlation observed in TGCT. This evidence concerns the gene BTLA and cancer.