CEACAM8 and Sepsis: The density gradient centrifugation of peripheral blood was performed to separate PBMC and neutrophil fractions using (Fig. 1A), and the flow cytometry gating from the PBMC fraction using CD66b (a marker for neutrophils) (Fig. 1B) demonstrated more prominent LDN (CD66b-positive cells in the PBMC) in the patients with sepsis compared with the non-sepsis (ICU) and healthy control (Fig. 1C).