To evaluate the role of Senataxin (SETX) in Myc-induced replicative stress (RS), we took advantage of the human osteosarcoma-derived U2OS-MycER cell line [37], which constitutively expresses the MycER transgene, encoding the Myc protein fused to the estrogen receptor (ER). This evidence concerns the gene ESR1 and osteosarcoma.