Identifying allergens in food proteinsusing classical methodsrelies on immunological, clinical, and physicochemical methods toevaluate IgE reactivity, immune response activation, and protein stability.31In vitro immunological assays,such as ELISA, Western blotting, the Basophil Activation Test (BAT),and the Radioallergosorbent Test (RAST), are commonly used to detectIgE binding and immune system recognition of specific proteins.40 These methods help determine whether a proteinhas the potential to trigger allergic reactions by examining interactionswith antibodies from allergic individuals. Here, IGHE is linked to allergic disease.